THERIOGENOLOGY AND CRYOBIOLOGY IN ENDANGERED SPECIES RECOVERY

Abstract

This paper will examine how theriogenology and cryobiology can assist in the reproduction of endangered species and their recovery.  We employed a well-organized trial protocol whereby we began the study by genomic profiling and gathering gametes and compared two different cryopreservation modes such as vitrification and slow-freezing.  To ensure the viable survival of the cells after thawing we employed CASA and oocyte scoring. More stable gametes were provided by vitrification.  Assisted reproductive technologies (ART) or assisted reproductive technology (ART) processes including IVF, ICSI and AI were used to produce embryos and implanted in surrogate, species-compatible with the embryonic's gestational age.  We monitored the growth and performance of the embryo and how they performed after pregnancy. Transformation to blastocyst was 78 percent and live birth 62 percent.  The reintegration methods such as genetic testing and GPS track demonstrated that the animals were able to procreate and acclimatize when released.  The best formula was using physiological and genetic measures that helped expertise to make determine predictions about gamete selection and outcomes of embryo viability.  Responses indicate that cryo-assisted ART pipeline can assist endangered species with reproductive issues, and this can serve as a prototype of the conservation process that could be scaled-up.